Meloidogyne chitwoodi

BioProject PRJNA666745 | Data Source Northwest Potato Research Consortium | Taxonomy ID 59747

About Meloidogyne chitwoodi

Meloidogyne chitwoodi, commonly known as the Columbia root-knot nematode (CRKN), is an important soilborne, sedentary endoparasite of potato. It is one of the most devastating pests of potato in the U.S. Pacific Northwest (PNW). Nematode-infected tubers develop external as well as internal defects, making the potato tubers unmarketable, and resulting in economic losses. M. chitwoodi can also infect tomato, sugar beet, wheat, and corn. It has a short life cycle (23 to 25 days), resulting in rapid population density build-up over a single cropping season.

Genome Assembly & Annotation

Assembly

For this assembly, M. chitwoodi race 1 population, extracted from 3-month-old infected tomato plants, was used. This population did not reproduce on alfalfa (used as a rotation crop to control M. chitwoodi populations).

According to Bali S, et al., (2021), PacBio long sequencing reads were assembled using the SMRT analysis pipeline. Short Illumina reads were used to error correct PacBio data contigs using pilon v.1.22.

Annotation

According to Bali S, et al., (2021), genome annotation was performed using the M. hapla protein dataset as Protein Homology Evidence and the SNAP training file prepared from the first round of annotation. EST evidence was used.

Key Publications

Assembly Statistics

AssemblyASM1518303v1, GCA_015183035.1
StrainRace1
Database VersionWBPS18
Genome Size47,477,280
Data SourceNorthwest Potato Research Consortium
Annotation Version2022-10-WormBase

Gene counts

Coding genes12,295
Gene transcripts12,304

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