Heterodera schachtii

BioProject PRJNA767548 | Data Source Wageningen University and Research | Taxonomy ID 97005

About Heterodera schachtii

Heterodera schachtii, also known as the beet cyst nematode or sugarbeet nematode, is a plant pathogenic parasite which can infect more than 200 plants including the model plant Arabidopsis thaliana. H. schachtii is an important agricultural pest on sugar beet and brassicaceous crops in its own right and is closely related to the soybean cyst nematode, H. glycines, which is one of the severely economically damaging plant-parasitic nematodes worldwide.

There is 1 alternative genome project for Heterodera schachtii available in WormBase ParaSite: PRJNA722882

Genome Assembly & Annotation

Assembly

For this assembly, a Heterodera schachtii IRS (a Dutch beet cyst nematode population) was used.

According to Joris J. M. van Steenbrugge et. al. (2022), raw long Oxford Nanopore reads were corrected to, in essence, merge haplotypes using the correction mode in CANU. Using wtdgb2 version 2.3, multiple initial genome assemblies were generated. The contigs from the assemblies were then improved using finishersc version 2.1. Gaps in the assemblies were then filled using gapfiller version 1.0. The resulting assembly was polished with ONT reads using MEDAKA v1.4.1, followed by five iterations of polishing with pilon version 1.23 using Illumina short reads.

Annotation

Using braker version 2.1.2, gene annotations were predicted for both assemblies at default settings outputting gff3 annotations and aided by RNAseq data of different life stages (NCBI Bioproject PRJNA767548). Full details on the generation of the genome assemblies and prediction of genes are available here. Read more at Joris J. M. van Steenbrugge et. al. (2022).

Key Publications

Assembly Statistics

AssemblyWUR_Hsch_IRS_1.0, GCA_020449115.1
StrainIRS
Database VersionWBPS18
Genome Size190,153,211
Data SourceWageningen University and Research
Annotation Version2022-09-WormBase

Gene counts

Coding genes29,851
Gene transcripts31,564

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