Anisakis simplex

BioProject PRJEB496 | Data Source Wellcome Sanger Institute | Taxonomy ID 6269

About Anisakis simplex

The nematode Anisakis simplex, or herring worm, is a parasite of marine mammals. Humans are incidental hosts through ingestion of undercooked infected fish, leading to anisakiasis.

Genome Assembly & Annotation

Assembly

The draft genome assembly was produced by the Parasite Genomic group at the Wellcome Trust Sanger Institute, in collaboration with Carmen Cuellar (Facultad de Farmacia. Universidad Complutense de Madrid) as part of the 50 Helminth Genomes project. The assembly uses Illumina paired-end sequencing followed by an in-house genome assembly pipeline comprising various steps, including contig assembly, scaffolding, gap-filling and error-correction.

Annotation

The gene predictions were made by the Parasite Genomics group at the Wellcome Trust Sanger Institute and WormBase, as part of the 50 Helminth Genomes project. An in-house pipeline was developed that used MAKER to generate high-quality annotations by integrating evidence from multiple sources: ab initio gene predictions from AUGUSTUS, GeneMark-ES, and SNAP; projected annotation from C. elegans (using GenBlastG) and the taxonomically nearest reference helminth genome (using RATT); and ESTs, mRNAs and proteins from related organisms aligned to the genome using BLAST, with refinement of alignments using Exonerate.

Key Publications

Assembly Statistics

AssemblyA_simplex_0011_upd, GCA_900617985.1
Database VersionWBPS18
Genome Size126,869,778
Data SourceWellcome Sanger Institute
Annotation Version2014-06-50HGPpatch

Gene counts

Coding genes20,971
Gene transcripts20,971

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